Mouse keratinocyte cultures
Isolation of epidermal keratinocytes from neonatal mice
TCA Precipitiation of proteins
Standard protocol for thawing of cells. Make sure it is suitable for your specific cell-type.
PAPS (phosphoadenosine phosphosulfate) preparation
This protocol is used to prepare PAPS, a sulfate donor used in for example assays measuring the activity of sulfotransferases.
Children's surgeon to present results of new anti-rejection protocol at World Transplant Congress
Children's Hospital of Pittsburgh Surgeon and Researcher Rakesh Sindhi, MD, to discuss new protocol that has shown to improve patient outcomes
Preparing for an influenza pandemic: A triage protocol in the face of limited resources
The recent outbreaks of avian influenza (H5N1) around the world have placed a renewed emphasis on preparing for an influenza pandemic in humans
Ottawa aggressive protocol for acute atrial fibrillation is successful
There is no consensus on Emergency Department management of acute atrial fibrillation (AAF) or atrial flutter (AAFL).
A frenzy of fruit fly methods featured in Cold Spring Harbor Protocols
For the past century, fruit flies—or Drosophila—have provided innumerable insights into the genetics and biology of development, learning and memory, behavior, vision, and other processes.
Isolation of murine splenocytes
In order to study spleen cells (e.g. lymphocytes, granulocytes, other immune cells), it helps to make single-cell suspensions so that the cells can be manipulated ex vivo easily.
Intracellular cytokine staining for flow cytometry (mouse)
Staining for intracellular cytokines, followed by flow cytometry analysis, can provide single-cell information about a cell population that one cannot obtain from surface staining or ELISA
Rice Researchers Develop First Fully Automatic Method To Track 3-D Movement Of Cancer Cells
Cancer researchers know the disease spreads from organ to organ in a non random pattern
Toward Pinpointing The Location Of Bacterial Infections
In an advance in the emerging field of bacterial imaging, scientists are reporting development of a method for identifying specific sites of localized bacterial infections in living animals.
Adenine 'Tails' Make Tailored Anchors For DNA
Researchers have demonstrated a deceptively simple technique for chemically bonding single strands of DNA to gold.
New Blood Test Could Detect Lung Cancer In Its Earliest Stages
Lung cancer is often deadly by the time doctors have detected it, but scientists at Duke University Medical Center are developing a non-invasive test that could detect lung cancer in its earliest stages, while it is still treatable.
Scientists Make First Step Towards 'Holy Grail' Of Crystallography
Scientists from Imperial College London and the University of Surrey have developed a new technique for crystallising proteins, a discovery which could help speed up the development of new medicines and treatments.
Optical Bioimaging: From Living Tissue to a Single Molecule: Single-Molecule Visualization of Cell Signaling Processes of Epidermal Growth Factor Receptor
Single-molecule analysis has proven to be a powerful technology to analyze intracellular protein systems
Importance of quantifying insulin secretion in relation to insulin sensitivity to accurately assess beta cell function in clinical studies
Insulin sensitivity and insulin secretion are mutually related such that insulin resistance is compensated by increased insulin secretion.
Mass Spectrometry as a Diagnostic and a Cancer Biomarker Discovery Tool
In this review, the author concentrated more on questions that cast doubt on the results reported and propose experiments to investigate these questions in detail, before the technique is used at the clinic.
Imaging of chlorophyll a fluorescence: theoretical and practical aspects of an emerging technique for the monitoring of photosynthetic performance
This paper discusses some of the theoretical and practical issues associated with the imaging of Chl a fluorescence and with Chl a fluorometry in general.
Metabolic Mapping of Proteinase Activity with Emphasis on In Situ Zymography of Gelatinases : Review and Protocols
In situ zymography provides data that extend our understanding of the role of specific proteinases in various physiological and pathological conditions.