Western blot analysis

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Western blot analysis

A procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pre-or post-tagged with a labelled secondary protein.

See: immunoblot.

Synonym: western blot, western blotting.

Origin: coined to distinguish it from eponymic Southern blot a.