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Southern blot

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southern blot

A technique used for searching for a specific dNA fragment. The process is as follows:

1. separate DNA fragments by gel electrophoresis

2. Change pH of gel to basic, thus allowing disruption of H-bonds

3. blot gel with nitrocellulose paper

4. Heat paper so as to fix dNA fragments

5. probe with labelled messenger RNA or cDNA

6. wash

complementary mRNA/cDNA fragments will have hybridised.