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Sepharose

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Definition/Description

A registered trademark owned by GE Healthcare companies for the bead-formed gel prepared from agarose, which is used as base matrix for several chromatographic products used for purification of proteins and other biomolecules.


Supplement

In its natural state agarose it occurs as part of the complex mixture of charged and neutral polysaccharides referred to as agar. The agarose used to make SepharoseTM is obtained by a purification process which removes the charged polysaccharides to give a gel with only a very small number of residual charged groups.

SepharoseTM is given different chromatographic properties by modification or attachment of a ligand.