Restriction site

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noun, plural: restriction sites

The site in a polynucleotide chain as to where the restriction enzyme cleaves nucleotides by hydrolyzing the phosphodiester bond between them.


The restriction enzyme recognizes the site as to where it cuts via a specific sequence of nucleotides along the polynucleotide chain. For example, when the restriction enzyme, Eco RI, recognizes the palindromic sequence GAATTC, it cuts between G and A on the top and bottom strands.


See also: restriction enzyme

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