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Resorcinol phthalic anhydride

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resorcinol phthalic anhydride --> fluorescein

(Science: chemical) Fluorophore commonly used in microscopy.

Fluorescein di acetate can be used as a vital stain or can be conjugated to proteins (particularly antibodies) using isothiocyanate.

excitation is at 365nm and the emitted light is green yellow (450-490nm). The emission spectrum is pH sensitive and fluorescein can therefore be used to measure pH in intracellular compartments.