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Radioimmunoelectrophoresis

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radioimmunoelectrophoresis

Immunoelectro phoresis in which the antigen or antibody is labelled with a radioisotope; e.g., in testing for insulin-binding antibodies by treating the test serum with radioactive iodine-labelled insulin, subjecting the mixture (antigen) to electrophoresis, precipitating the separated immunoglobulins with immunoglobulin-specific antiserum, and, then, with radiosensitive film (autoradiography), testing for bound insulin in the precipitates.