Confused and have questions? We’ve got answers. With Chegg Study, you can get step-by-step solutions to your questions from an expert in the field. If you rather get 1:1 study help, try 30 minutes of free online tutoring with Chegg Tutors.

Periodic acid schiff reaction

From Biology-Online Dictionary | Biology-Online Dictionary
Jump to: navigation, search

periodic acid Schiff reaction

(Science: technique) A histochemical technique based on periodic acid oxidation of a substance containing the 1,2-glycol grouping.

It is used for staining carbohydrates as the resulting dialdehyde reacts with Schiff reagent to form a coloured product.

The adjacent hydroxyl groups are oxidized to form aldehydes by periodic acid (HIO4) and these aldehyde groups react with Schiff's reagent (basic fuchsin decolourised by sulphurous acid) to give a purple colour.

Acronym: PAS