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Orientation chamber

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orientation chamber

(Science: cell culture) chamber designed by Zigmond in which to test the ability of cells (neutrophils) to orient in a gradient of chemoattractant. The chamber is similar to a haemocytometer, but with a depth of only ca. 20m. The gradient is set up by diffusion from one well to the other and the orientation of cells towards the well containing chemoattractant is scored on the basis of their morphology or by filming their movement.