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From Biology-Online Dictionary | Biology-Online Dictionary
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1. A wife or a widow, especially, one who has borne children; a woman of staid or motherly manners. Your wives, your daughters, Your matrons, and your maids. (Shak) Grave from her cradle, insomuch that she was a matron before she was a mother. (Fuller)

2. A housekeeper; especially, a woman who manages the domestic economy of a public instution; a head nurse in a hospital; as, the matron of a school or hospital. Jury of matrons, a jury of experienced women called to determine the question of pregnancy when set up in bar of execution, and for other cognate purposes.

Origin: f. Matrone, L. Matrona, fr. Mater mother. See mother.