Layilin is newly cloned 55 kDa membrane binding colleague for talin that is extensively expressed in different cell types and tissue extracts and has 280-435 amino acids where it is generally located at the peripheral ruffles of spreading cells and is recruited to membrane ruffles in cells. It is then represents as membrane-binding sites that recruit talin to ruffles and is mapped on chromosome 11q23.1.
Layilin extracellular domain is homologous with the carbohydrates-recognition domains of C-type lectins. It functions in a way analogous to selectins in mediating transient interactions with extracellular matrix. It is also a key regulator of cytoskeleton plasma membrane interactions in polarized cells that involved in maintaining intercellularepithelial contact.
Layilin is a surface hyaluronan receptor with an extracellular domain that interacts with merlin, radixin and moesin family. Studies using microtiter plate binding assays, co-precipitation and staining techniques reveals that this protein is specifically binds to hyaluronan but not to other glycosaminoglycans. Hyaluronan, an omnipresent extracellular matrix component divulges a remarkable similarity between layilin and CD44 since both can bind to cytoskeleton-membrane linker proteins through their cytoplasmic domains.
Gene name: LAYN
Protein name: Layilin