In situ hybridization

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A type of hybridization that detects and localizes the presence of specific DNA sequence within tissue samples (‘’in situ’’) when a labeled DNA or RNA probe binds with it by complementary base pairing.


DNA ISH can be used to determine the structure of chromosomes. Fluorescent DNA ISH (FISH) can, for example, be used in medical diagnostics to assess chromosomal integrity. RNA ISH (hybridization histochemistry) is used to measure and localize mRNAs and other transcripts within tissue sections or whole mounts.

‘’Abbreviation:’’ ISH

‘’Alternative form:’’ ‘’in situ’’ hybridisation

‘’Related terms:’’