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Fluorescein

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Fluorescein

(Science: chemical) Fluorophore commonly used in microscopy.

fluorescein di acetate can be used as a vital stain or can be conjugated to proteins (particularly antibodies) using isothiocyanate.

excitation is at 365nm and the emitted light is green yellow (450-490nm). The emission spectrum is ph sensitive and fluorescein can therefore be used to measure ph in intracellular compartments.