Dye exclusion test

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Dye exclusion test

a test to determine cell viability in which a dilute solution of certain dyes (e.g., trypan blue, eosin Y, nigrosin, Alcian blue) is mixed with a suspension of live cells; cells that exclude dye are considered to be alive while cells that stain are considered dead; it is not always an accurate test because it indicates only the structural integrity of the cell membrane.