Dot blot

From Biology-Online Dictionary
Jump to: navigation, search

Dot blot

(Science: molecular biology, procedure) method for detecting a specific protein or message. A spot of solution is dotted onto nitrocellulose paper, a specific antibody or probe is allowed to bind and the presence of bound antibody/probe then shown by using a peroxidase coupled second antibody, as in western blotting or by other visualisation methods.