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Dna sequence analysis

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Dna sequence analysis

(Science: molecular biology) determination of the nucleotide Sequence of a length of dna. Typically, this is performed by cloning the dna of interest, so that enough can be prepared to allow the sequence to be determined, usually by the Sanger dideoxy chain temination or Maxam gilbert chain degradation techniques. The resulting reactions are then run on a large sequencing gel, capable of resolving single nucleotide differences in chain length. Recently, pcr based methods have obviated the need to clone the dna under some conditions and automated dna sequencing has become widely available.