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Boyden chamber asssay

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An assay used for studying cell migration and cell invasion by means of a Boyden chamber, a device made up of two compartments separated by a microporous membrane in order that cells in a suspension loaded into the first compartment may pass through towards the other compartment containing chemoattractants, and after a given incubation period the number of cells that migrated underneath the membrane is determined


The Boyden chamber assay can be used to determine or study cell migration, such as leukocyte chemotaxis and cancer cell migration.

Also called:

  • filter membrane migration assay
  • trans-well migration assay
  • chemotaxis assay

See also:

Related term(s):