Bandshift assay

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Bandshift assay

(Science: investigation) An assay for proteins, such as transcription factors, that bind specific dna sequences.

a labelled oligonucleotide corresponding to the recognition sequence is incubated with an appropriate nuclear protein extract and run on a nondenaturing acrylamide gel. Oligonucleotides that have been bound by proteins are retarded relative to those that are unbound.