Avidin-biotin immunofluorescence

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Avidin-biotin immunofluorescence is an Immunohistochemical assay wherein the initial step is to label antibodies with biotin. Biotin is a naturally water-soluble B vitamin involved in many metabolic processes, such as in the utilization of amino acids, carbohydrates, and fats. Soon, biotin was used in assays and biotechnology as a protein conjugate. Because it has a high affinity and specificity for avidin, it is used in this assay in order to determine and isolate biotinylated proteins of interest (in this case, antibodies). Avidin can bind up to four molecules of biotin all at the same time. Fluorescent-labeled avidin is added for the subsequent fluorescent microscopy. Avidin-biotin immunofluorescence is used to detect the presence of certain complexes. Abbreviation: ABIF

See also


  1. Overview of Protein Labeling | Thermo Fisher Scientific. (2013). Retrieved from Thermofisher.com website: https://www.thermofisher.com/ph/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/overview-protein-labeling.html#/legacy=www.piercenet.com

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