Stuck on a problem? Get great tutoring at an affordable price with Chegg. Click here to join and get your 1st 30 minutes Free!

Affinity chromatography

From Biology-Online Dictionary
Jump to: navigation, search

Affinity chromatography

(Science: investigation) a technique of analytical chemistry used to separate and purify a biological molecule from a mixture, based on the attraction of the molecule of interest to a particular ligand which has been previously attached to a solid, inert substance.

The mixture is passed through a column containing the ligand attached to the stationary substance, so that the molecule of interest stays within the column while the rest of the mixture continues through to the end. Then, a different chemical is flushed through the column to detach the molecule from the ligand and bring it out separately from the rest of the mixture.