cloning 5kb insert

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jamiejlg
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cloning 5kb insert

Post by jamiejlg » Tue Feb 27, 2007 6:37 pm

Hello, I have to clone a gene that is nearly 5kb. Can anyone suggest a specific vector that can handle larger fragments?

Vagabond
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Post by Vagabond » Tue Feb 27, 2007 6:46 pm

I used pblue script to clone a 12kb fragment..
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You know dog spelled backwards is god.
Coincidence? I think not. . .

SororSaudade
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Post by SororSaudade » Tue Feb 27, 2007 10:20 pm

avoid at all costs blunt end ligation :P

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