protein concentration and SDS-Page ques.

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jamiejlg
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protein concentration and SDS-Page ques.

Post by jamiejlg » Tue Feb 27, 2007 6:30 am

Hi- I'm new to protein work and looking for guidance. I have proteins in 10 mM HCl at a concentration of 3mg/ml. I need to load 2 and 4 ug onto an SDS Page. Can anyone help me calculate that? How do I denature it?
Also- will the HCl have an imapct on the results- do I have to neutralize?

Thanks in advance for your help and feel free to contact me reguarding all things DNA.

Vagabond
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Post by Vagabond » Tue Feb 27, 2007 1:19 pm

3 mg /ml is 3000 ug/ml or 3 ug/ul. You will need to add a denaturing buffer and then boil the sample prior to loading the gel. I an not sure about neutralizing the HCl the denaturing/loading buffer may take care of it for you.
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