inserting plasmids into bacteria vs mammalian cells

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physio_kid
Garter
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inserting plasmids into bacteria vs mammalian cells

Post by physio_kid » Mon Feb 26, 2007 1:24 pm

Hi--- I have a quick question that has been bothering me for some time. If you want to express a specefic protein, why can't you use bacteria to express it? Maybe you can? is it because it is not as efficient as mammalian cells? Say I want to study elastin or something......

I guess my question is: what are the advantages of inserting plasmids (for protein expression) into bacteria vs mammalian cells?

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ping
Coral
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Post by ping » Mon Feb 26, 2007 1:30 pm

Bacteria not always process the proteins properly,so they are not functional.Mammalian proteins are often glycolized and cannot be expressed in bacterial cells.

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