lysosomes

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sonken
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lysosomes

Post by sonken » Tue Feb 20, 2007 3:35 pm

I got three questions regarding the property of lysosome. I Hope someone can help me find the answers. Thank you so much!

1. The lumen of lysosome has a pH of 4.6, but How would you measure the pH of lysosomal lumen?

2. What is the advantage to the cell in maintaining a acidic pH in the lumen of the lysosome?

3. How do lysosomes protect the integrity of their membranes from attack by the lysosome enzymes?

x2eagle18
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Post by x2eagle18 » Tue Feb 20, 2007 10:02 pm

With regard to your second question:

Since lysosomal hydrolases are only active in an acidic environment such as the lumen of lysosomes, they cannot degrade protein in other parts of the cell. If a lysosome were to rupture then those hydrolases would be free to destroy proteins throughout the cytoplasm, but since they are not enzymatically active at a neutral pH, they cannot do any damage. Additionally, after the enzymes are synthesized, they must be transported to the lysosome. Therefore, there must be a mechanism to prevent them from degrading protein during this trafficking.

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MrMistery
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Post by MrMistery » Tue Feb 20, 2007 11:17 pm

3. look for glycosilation of proteins in regard to protecting them from proteases.

1. Absolutely no idea.
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Vagabond
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Post by Vagabond » Wed Feb 21, 2007 2:39 pm

Here is a quick journal article for you to start you on your journy

http://www.springerlink.com/content/t926866648p8734q/
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Dustfinger
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Post by Dustfinger » Mon Feb 26, 2007 10:28 am

1. Can't they be extracted ? And then you could measure the PH.
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