Why circular plasmid is preferred?

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satrohraj
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Why circular plasmid is preferred?

Post by satrohraj » Sun Feb 18, 2007 12:19 pm

Why circular plasmid is preferred to linear plasmid?
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Locus
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Post by Locus » Sun Feb 18, 2007 2:35 pm

I know two reasons:
- linear plasmide is sensitive to DNAase that must be within the cell
- circular plasmid have not problems with duplication - all known pherments can not duplicate end of nucleic acid. so, we need mechanis like telomerase.
Evolution will arrange everything

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MrMistery
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Post by MrMistery » Sun Feb 18, 2007 3:04 pm

yes, linear plasmids would be chopped up by exonucleases within bacterial cells.
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

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