deglycosylation

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metclef
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deglycosylation

Post by metclef » Thu Feb 15, 2007 4:29 pm

Hi, could anyone tell me a protocol to deglycosylate oligo mannose units linked to aspargene moeities in a glycoprotein..without denaturing the protein. All my protocols I can put my hands on till now denature the protein and I hence cannot work with the deglycosylated protein anymore. Its like a nightmare now!!

scmesser
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Post by scmesser » Sat Mar 10, 2007 7:58 pm

Hi, could anyone tell me a protocol to deglycosylate oligo mannose units linked to aspargene moeities in a glycoprotein..without denaturing the protein. All my protocols I can put my hands on till now denature the protein and I hence cannot work with the deglycosylated protein anymore. Its like a nightmare now!!

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