Polyermase Chain Reaction Question

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Vagabond
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Joined: Mon Feb 19, 2007 7:01 pm

Post by Vagabond » Wed Feb 21, 2007 4:23 pm

Ah sorry i missed that last question...

Look at my above post with the sequences. It may be hard to see so you may need to either write it out or use a text program but i will try an type (talk) you through it.

Line up your original sequences so they base pair (think of this as your template).

Now heat up your sample - breaks base pairs and you are left with two single strand DNA molecules (just add some returns (spaces) between the two original sequences (your templates)).

Ok now add in your primers one will bind to one strand and the other will bind to the strand. (they will be facing each other). Now add in your Taq and reduce your temp and (if you added dNTPs and a buffer) off it goes amplifying. repeat temp cycles and you will eventually get short DNA molecules which start and end with your primers.

Hope that helps.
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You know dog spelled backwards is god.
Coincidence? I think not. . .

Agronomy
Garter
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Joined: Wed Jan 17, 2007 5:04 am

Post by Agronomy » Wed Feb 21, 2007 5:28 pm

hmm, I still don't understand...would u be able to write out the sequence and then BOLD what you are talking about...i'm sry I am really slow at undersatnding this

Vagabond
Death Adder
Death Adder
Posts: 59
Joined: Mon Feb 19, 2007 7:01 pm

Post by Vagabond » Wed Feb 21, 2007 5:40 pm

ok see if this pic helps also check out this animation http://www.maxanim.com/genetics/PCR/PCR.htm
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Primer binding sequence
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You know dog spelled backwards is god.
Coincidence? I think not. . .

Agronomy
Garter
Garter
Posts: 29
Joined: Wed Jan 17, 2007 5:04 am

Post by Agronomy » Wed Feb 21, 2007 5:53 pm

Thank you for all your help :-)

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