water quality control

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participant
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water quality control

Post by participant » Wed Feb 07, 2007 4:10 pm

Apparently, there's much more to DI water than we thought. My PI and I have been having trouble with our cells and one of her colleagues blames the water from the DI tanks so she asked me to get second opinions from different people.

Does that sound right to you? If we use the DI tank water (and autoclave it) should we also be doing endotoxin-testing w/ every batch of PBS we make? What about Millipore/Ultrapure type apparatuses? How do you guys go about quality control?

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imreally
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Post by imreally » Thu Feb 08, 2007 11:15 am

reasonable.
and make sure your water is fresh
Nothing.

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canalon
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Post by canalon » Thu Feb 08, 2007 1:40 pm

Don't do QC, But I hate those big tanks because I have seen algae growing in some. Fresh production would be better and safer and less likely to be contaminated.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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LilKim
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Post by LilKim » Fri Feb 09, 2007 1:33 am

In one lab... we used to buy our water (we had alot of money)

In my current lab, we use a millipore system and it has been fine for PCR and cell culture stuff. Our new lab techn. recently began to autoclave the millipore stuff for cell culture and haven't had any problems with our cells.

water is such of pain sometimes... good luck though

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