PCR primers

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Atique
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PCR primers

Post by Atique » Wed Feb 07, 2007 5:14 am

Hi everyone,
Can anybody let me know is there any software or database from where we can know whether the primers which we have selected are the correct primes for our gene of interest??
Please give suggestions excluding seaching for literatures.
Regards
Atiq

hara
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Post by hara » Wed Feb 07, 2007 3:56 pm

go to google then pubmed then entrez pubmed then nucleotide then blast then nucleotide-nucleotide(blastn) and then you put the sequence of the primer....try it and tell me if you suceed or else i help you...

epsilonbeta
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e pcr

Post by epsilonbeta » Wed Feb 07, 2007 7:55 pm

You can do e-PCR ...another alternative...
link:

http://www.ncbi.nlm.nih.gov/sutils/e-pc ... m=0&gaps=0


put yr forward and reverse primer..and the expected size... and you should get a hit with it...

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