Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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Agronomy
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by Agronomy » Wed Jan 31, 2007 5:32 pm
Is lambda in its circular or linear form after digested with Bam HI?
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MrMistery
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by MrMistery » Wed Jan 31, 2007 6:11 pm
anything is linear after you digest it with something..
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter
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canalon
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by canalon » Wed Jan 31, 2007 6:53 pm
MrMistery wrote:anything is linear after you digest it with something..
Provided there is at least one restriction site for the said enzyme in your sequence. Of course
Patrick
Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
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MrMistery
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by MrMistery » Wed Jan 31, 2007 8:16 pm
oh yes that is so foolish of me. Of course, you need to add an enzyme that will actually digest your DNA, not just stand there... Partick is right as usual
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter
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SororSaudade
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by SororSaudade » Wed Jan 31, 2007 9:02 pm
Agronomy, I believe you can easily find a restriction map of that plasmid on the web, so that you can answer your question
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Caspase
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by Caspase » Thu Feb 01, 2007 12:04 am
MrMistery wrote:anything is linear after you digest it with something..
Good answer, a bit dry though.
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MrMistery
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by MrMistery » Fri Feb 02, 2007 5:49 pm
Well it's a straight to the point answer. I could have replied "Yes"
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter
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