lipase enzyme

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kashfi
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lipase enzyme

Post by kashfi » Wed Jan 31, 2007 4:16 pm

hi!
i'm doing an experiment where i have to change the substrate concentration which is milk and i want to know how it will affect the lipase enzyme.
can anyone help ME and also if you know a good website for enzyme post it to me.
THANKS

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MrMistery
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Post by MrMistery » Wed Jan 31, 2007 6:21 pm

Well i can tell you this: milk contains a fairly small amount of fat. if you diluted that amount will decrease still further. So, by measuring enzyme activity at different substrates you should get a nice MM plot...
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kashfi
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re:lipase enzyme

Post by kashfi » Thu Feb 01, 2007 8:34 am

what is the mm plot you were talkin about

sdekivit
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Post by sdekivit » Thu Feb 01, 2007 8:30 pm

probably michaelis-menten ? but when i determine enzyme activities, i draw an absorption curve with a spectrophotometric assay.

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MrMistery
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Post by MrMistery » Fri Feb 02, 2007 5:38 pm

yeah you can do that, i was thinking that you actually want to study your enzyme. Sdekivit's method is the best way to go: use a dye that reacts with the product of your enzyme catalysed reaction. The more product, the more dye. Then measure absorbtion ratio, do a graph and(if you did the work properly) you would a straight line. If not, you will get 16/40 at your biochemistry exam like me.
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

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