HUVEC-C (how can i mantain a confluece culture??)

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wheatonhgc
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HUVEC-C (how can i mantain a confluece culture??)

Post by wheatonhgc » Sat Jan 27, 2007 5:38 am

HUVEC!!!! Hi All, i have been having a lot of trouble culturing HUVEC-C into confluency... I ordered the cells from ATCC. I modified the F-12 Kaigh's medida with 10%fetal bovine serum, 0.8% penicillin, 0.1mg/ml heparin, and 0.05 mg/ml ECGS. Also, cells are incubated at 37C with 5%CO2.. I don't know why my cells died within two days. Please help me out if you know the cause of cell death or if you know who is the expert on this cell type, please email me at [email protected]

thank you for your help!!!!

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