loading buffer

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hara
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loading buffer

Post by hara » Wed Jan 17, 2007 1:56 pm

why should i have a loading buffer that contains sds?
what is the difference with a loading buffer that contains just xylene cyanol FF,bromophenol blue, glycerol, EDTA,ddH2O?
if someone knows exactly the concetrations of the previous recipe i would like to let me know..thanks

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canalon
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Post by canalon » Wed Jan 17, 2007 4:12 pm

For what? There are a lot of recipe of loading buffer, google and you will find all details. Adding SDS is usually only for loading proteins (to denature them) is that what you are looking for?
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

SororSaudade
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Post by SororSaudade » Wed Jan 17, 2007 4:17 pm

http://www.thelabrat.com/protocols/XCBBDNAload.shtml

I do it without SDS for DNA electrophoresis.

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