Hypophysis removal

Debate and discussion of any biological questions not pertaining to a particular topic.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
User avatar
Dr.Stein
King Cobra
King Cobra
Posts: 3501
Joined: Thu Jul 07, 2005 7:58 am
Location: 55284 Yogyakarta, Indonesia
Contact:

Hypophysis removal

Post by Dr.Stein » Tue Jan 16, 2007 11:49 am

This old lady got problem on how to remove hypophysis from its place for then to be processed for histological preparation. For your information, the animal is three-month-old white Balb/c mouse. As you may know, this strain is a tiny cuty thingy mousy :?

Thank you for your information 8)

Protocol/steps or pics would be nice :)
Image

User avatar
sachin
King Cobra
King Cobra
Posts: 1517
Joined: Mon Jan 30, 2006 12:39 pm
Location: MUMBAI / INDIA
Contact:

Post by sachin » Tue Jan 16, 2007 2:52 pm

Remove Hypophysis means?
Last edited by sachin on Wed Jan 17, 2007 2:02 pm, edited 1 time in total.
Senior Education Officer, BNHS, India. www.bnhs.org

Bitter Truth!
Who says reason for world war III will be Petrol?
Reason lies in two words "Me and Mine".

User avatar
MrMistery
Inland Taipan
Inland Taipan
Posts: 6832
Joined: Thu Mar 03, 2005 10:18 pm
Location: Romania(small and unimportant country)
Contact:

Post by MrMistery » Tue Jan 16, 2007 4:30 pm

she means remove the pituitary gland surgically...
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

User avatar
Dr.Stein
King Cobra
King Cobra
Posts: 3501
Joined: Thu Jul 07, 2005 7:58 am
Location: 55284 Yogyakarta, Indonesia
Contact:

Post by Dr.Stein » Wed Jan 17, 2007 5:45 am

Andrei is correct. Because the gland is a tiny baby, super care must be taken to remove it out. However, when the removal is successful, we still need to process for preparation. This means we will face another problem because organs/tissues sometime get shrinked in fixative or another chemicals in the process. I cannot imagine if that tiny baby will be shrinked. I will get nothing to examine by then :(
Image

User avatar
sachin
King Cobra
King Cobra
Posts: 1517
Joined: Mon Jan 30, 2006 12:39 pm
Location: MUMBAI / INDIA
Contact:

Post by sachin » Wed Jan 17, 2007 2:06 pm

That's the major problem most of the time....
But can you tell which method you are using currently for that; may be we can help you....
Senior Education Officer, BNHS, India. www.bnhs.org

Bitter Truth!
Who says reason for world war III will be Petrol?
Reason lies in two words "Me and Mine".

User avatar
Dr.Stein
King Cobra
King Cobra
Posts: 3501
Joined: Thu Jul 07, 2005 7:58 am
Location: 55284 Yogyakarta, Indonesia
Contact:

Post by Dr.Stein » Thu Jan 18, 2007 9:31 am

Routine histological method: paraffin method with fixative formaldehyde and standars PAS staining for beta cell.
Image

Post Reply

Who is online

Users browsing this forum: Google Adsense [Bot] and 9 guests