Question on sequencing

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aaroncjj_2020
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Question on sequencing

Post by aaroncjj_2020 » Mon Jan 15, 2007 3:27 am

i've got a result from my sequencing. when i blast it (eg. aattgcg), it is not related to the spesies that i wan. when i blast it reversely (eg. gcgttaa), it shows that it is related to my spesies. how can we explain it?

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mith
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Post by mith » Mon Jan 15, 2007 1:53 pm

inversion mutation
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Post by sachin » Mon Jan 15, 2007 2:49 pm

Or sequence may be in that manner.......? I guess...
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aaroncjj_2020
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Post by aaroncjj_2020 » Tue Jan 16, 2007 2:08 am

if it is a an inversion mutation, how can the primers bind the sequence correct during RT-PCR as the 5' and 3' is already diff? thanks

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