Cre-Lox system

Genetics as it applies to evolution, molecular biology, and medical aspects.

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Joohn
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Cre-Lox system

Post by Joohn » Mon Jan 08, 2007 4:57 pm

In the case of spatio-temporally controlled targeted somatic mutagenesis in the mouse, 2 different mice reproduce themselves. One of them has been genetically modified by the homologuous recombinatory system and therefore possesses for example one exon situated between 2 Lox loci , exon we want to remove by crosslinking the mouse by another mouse which in the same way, got a cell-specific promotor controlled Cre-ERT gene. Knowing that the main reason for being of the Cre-Lox system is to prevent for example lethality in the embryo, how can one be sure that the genetically engeneered Cre mouse is viable?

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LilKim
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Post by LilKim » Tue Jan 09, 2007 7:04 am

I'm not sure if I get the question... but, if you express the cre (external induction such as tetrocycline... or endogenous induction by the promoter upstreatm of cre) and the mouse is viable and normal.. and healthy. I think it would be safe to assume that the cre gene is not lethal to the mouse carying the cre recombinase gene.

does this make sense?

Joohn
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Post by Joohn » Wed Jan 10, 2007 5:24 pm

I think I found the answer, thanks!

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