M13 vector

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ping
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M13 vector

Post by ping » Sun Dec 24, 2006 8:06 am

Is it true that you can clone up to 500-600nt when you use M13 as a vector?

wbla3335
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Post by wbla3335 » Wed Dec 27, 2006 11:49 am

I don't know what the largest insert on record is, but Maniatis says 40+ kilobases is possible. One nice thing about the M13 vectors is that they produce single-stranded DNA, which has provided the best quality sequence I have ever seen, even with 32P back in the old days.

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Post by ping » Wed Dec 27, 2006 12:33 pm

Thank you for the info, wbla3335

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