what is a restriction enzyme?

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
smrt guy
Garter
Garter
Posts: 1
Joined: Fri Dec 01, 2006 12:10 am

what is a restriction enzyme?

Post by smrt guy » Fri Dec 01, 2006 12:20 am

i have a quistion about restriction enzymes.what is it how can it be used and what is the procces of recombinant? :?: :?

User avatar
asutoshsahu
Death Adder
Death Adder
Posts: 98
Joined: Tue Jun 28, 2005 7:41 am
Location: India
Contact:

Post by asutoshsahu » Fri Dec 01, 2006 5:06 am

Restriction enzymes like endonuclease, ECO R1 cut DNA at particular sequences like GAATTC ..
Then this DNA fragment can be inserted to a plasmid which can be transfered to a vector( bacteria ) to multiply it and to produce a gene library.

sdekivit
King Cobra
King Cobra
Posts: 586
Joined: Sat Jul 30, 2005 7:16 pm
Location: holland
Contact:

Post by sdekivit » Fri Dec 01, 2006 7:11 pm

usually they cleave DNA at so called palindromes. Recall that besides cohesive ends, blunt ends can also be generated, which are more difficult to ligate in a plasmid.

Post Reply

Who is online

Users browsing this forum: No registered users and 4 guests