alternatives to generalized phage transduction?

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schwartz
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alternatives to generalized phage transduction?

Post by schwartz » Wed Nov 29, 2006 6:11 pm

I have been trying to transduce my recipent strains with mutations from the donor strain, but I don't get any colonies. I have checked plate concentrations, I have remade my phage lysates and even tried other strains. Even if there are four or five colonies, they don't have the mutation.

Does anyone know other ways to get a mutation from one strain into another? (this is a mutant caused by insertion of a transposon).

I need to get this working soon, please help! :cry: Thank you!

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