Tween

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Dr.Stein
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Tween

Post by Dr.Stein » Wed Nov 29, 2006 1:52 am

Hello, I am Dr.Stein ;)

I need a reference, or based to your own experience, of Tween concentration used to emulsify 70% ethanolic extract into distilled water. I have Tween 20 (10% stock). This extract will be administered orally as much as 0.5 mL to mouse (30 gram weight) for 34-67 days. Thank you :)

Please reply me ASAP! :lol:
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Dr.Stein
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Post by Dr.Stein » Wed Nov 29, 2006 9:05 am

None? :cry: :cry:
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Post by Dustfinger » Wed Nov 29, 2006 9:14 am

What is Tween ?
I don't understand your question.....
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Post by jnkdna » Thu Nov 30, 2006 2:02 pm

me neither. though i doubt i cud help u. i can always try!
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Post by Dr.Stein » Fri Dec 01, 2006 1:32 am

Tween is a harmless detergent for laboratory use with a pH balance about 6.8-7.2. It is applied to rinse something like chemicals, antibodies, and another liquids out from our tubes, plates, wells, or another places. It also is applied to emulsify lipids or any alcohol-based stuff like ethanolic extracts into aqueous environment, so it will dissolved completely. In our body system, we have bile to do this emulsion ;)
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Dustfinger
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Post by Dustfinger » Fri Dec 01, 2006 9:19 am

Then I'm sorry I don't know the answer......
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Post by Dr.Stein » Fri Dec 01, 2006 2:42 pm

It's alright :) Luckily, my 70% ethanolic extract is completely dissolved on the distilled water, so that I didn't need tween to emulsify ;) However, I am still expecting any asnwers to anticipate if in the future I have extract that is not easily dissolved :D
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Post by canalon » Fri Dec 01, 2006 4:22 pm

I used it at 0.02% but it was more as a wettig agent than an emulsifying agent. I might suggest that you try to find something that is hard to dissolve in water but does in ethanol (oil for example should do at low concentration) and make serial dilutions of tween and see which is the lowest concentration that works.

Since you work with a worst case scenario (oil is completely insoluble in water) it should work with anything else. don't you think?
Patrick

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any proof. (Ashley Montague)

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Post by boy_interrupted » Wed Dec 13, 2006 1:19 am

How about using Sodium Taurocholate? Or is it too strong?
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Post by Dr.Stein » Wed Dec 13, 2006 10:57 am

I never use it, have no experience yet. I am afraid my bioactive substance will react or at least change into something inactive with that :roll: Tween is neutral :)
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