enzymes in viruses

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lherzog
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enzymes in viruses

Post by lherzog » Mon Nov 27, 2006 11:11 pm

Help please!
my teacher gave me this question as homework:
A researcher inserted a gene from the pancreas of an elephant into a plasmid which was taken up by a bacterium. The bacterium then transcribed this gene into mRNA and translated the mRNA into protein. THe protein produced was useless; it contained many more amino acids than the protein made by the eukaryotic cell, and the amino acids were in a different sequence. Explain why. What enzyme found in some viruses could have solved this problem? Explain how the researcher could have used this viral enzyme to produce the protein she wanted.
please help me in any way that you can!
thanks

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fluktuacia
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Post by fluktuacia » Tue Nov 28, 2006 1:00 pm

doesn't it have something to do with introns and extrons (the introns were not spliced out from transcribed DNA asi it happends in the eukaryotic cells, because bacteria are prokaryotic)..
just a suggestion

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rob3
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Post by rob3 » Tue Nov 28, 2006 1:18 pm

I agree; the intons are still present in the elephants DNA, since the bacteria is a prokaryote, it has no mechanism for removing the introns from the pre-mRNA. Im not sure of the enzyme that would be used to remove them, maybe it is some sort of restriction enzyme? A way that the whole problem could be bypassed is by using cDNA from the elephant, as this is made from mRNA which contains no introns.

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MrMistery
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Post by MrMistery » Tue Nov 28, 2006 7:27 pm

i think it refers to the enzyme proteinase, if i remember correctly
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canalon
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Post by canalon » Tue Nov 28, 2006 9:56 pm

Certainly not. It refers to the enzyme found in the retrovirus that could definitely help getting rid of the introns problem...
Patrick

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any proof. (Ashley Montague)

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Post by MrMistery » Wed Nov 29, 2006 11:52 am

isn't that called proteinase? what's it called then? my memory fails me
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rob3
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Post by rob3 » Wed Nov 29, 2006 12:23 pm

Are you talking abou reverse transcriptase? Because I dont think that removes introns.

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canalon
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Post by canalon » Wed Nov 29, 2006 1:43 pm

Yes it is RT. It does not remove introns but it allows the synthesis of cDNA from the mRNA whose introns were removed
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rob3
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Post by rob3 » Wed Nov 29, 2006 6:39 pm

Yes, but werent we trying to find an enzyme present in viruses that COULD remove introns?

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Post by MrMistery » Wed Nov 29, 2006 6:57 pm

of yes, proteinase acts on proteins(duh)
and since the substrate for reverstranscriptase is mRNA it does not have introns.
sometimes i amase even myself at how dumm i am
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canalon
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Post by canalon » Wed Nov 29, 2006 7:50 pm

rob3 wrote:Yes, but werent we trying to find an enzyme present in viruses that COULD remove introns?


No, one that could solve the problem which was the presence of introns. If instead of cloning the whole gene, you clone the cDNA produced by the RT you have solved the problem with a viral enzyme.
Patrick

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