Perfusion embryonic mouse

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brain
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Perfusion embryonic mouse

Post by brain » Thu Nov 23, 2006 2:18 am

Hi,
I would like to open this topic to share the information! Please comment to me your experience in perfusion embryonic mouse. I now perfused E12, E14, E16, and E18 mice brain. I perfused mother first and cut the baby in PFA 4%. But I do not know the time to incubate? 2-4hours or more?
Thanks for your time

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Dr.Stein
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Post by Dr.Stein » Thu Nov 23, 2006 5:34 am

What will you do with those mice? I mean the duration of incubating or the time to apply this and that can be different depends on the purpose :?
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