Virus Titer

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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Krypie
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Virus Titer

Post by Krypie » Wed Nov 22, 2006 9:16 pm

I have produced virus from Phoenix gag pol cells. I have viral supernatant stocks frozen down. I wanted to quantify virus titer. What would be the best approach.

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canalon
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Post by canalon » Wed Nov 22, 2006 10:55 pm

Plaque formation if your virus lyse the cells it infects (mix different dilutions of your supernatant on top of a lawn of cell and count the lysis plaque per ml of solution considering the dilution)

Otherwise I do not have a clue.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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