Cloning SEKDEL by PCR

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SororSaudade
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Cloning SEKDEL by PCR

Post by SororSaudade » Thu Nov 16, 2006 3:56 pm

Hi everybody!

Has anyone ever tried to clone the sequence SEKDEL (for ER directioning) by PCR?
I was wondering if I could get some help with the primer design and some other technical issues (a link or a paper where i can find some decent description of the procedure would also be very very helpful).

Thanks a lot :roll:

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