DNA from Drosophila salivary gland squash slides

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davidallenjohnson
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DNA from Drosophila salivary gland squash slides

Post by davidallenjohnson » Wed Nov 15, 2006 7:12 am

I am trying unsuccessfully to isolate DNA from aceto orcein stained Drosophila salivary gland squashes that were fixed in acetic acid and a coverslip mounted with Coverbond (toluene soluble). They are old slides (about 30 years). I have soaked in toluene, then washed in alcohol:toluene, then alcohol. After that, I have tried the Pinpoint slide kit and also scraping off tissue and extracting DNA with the Promega Wizard DNA isolation kit. Is this DNA just too old and or degraded by the slide-making process and time? Any suggeestions?

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