Adv and Disadv of GFP over immunofluorescence

Discussion of all aspects of cellular structure, physiology and communication.

Moderators: Leonid, amiradm, BioTeam

Post Reply
Posts: 5
Joined: Sat Mar 18, 2006 10:20 pm
Location: Cyprus

Adv and Disadv of GFP over immunofluorescence

Post by medbio » Mon Nov 13, 2006 11:22 pm

Hey! Can anyone tell me advantages and disadvantages of using the GFP-fusion protein over immunofluorescence microscopy?

If someone knows please let me know!.. Your help is much appreciated! Thanks!
cArpE diEm

User avatar
Inland Taipan
Inland Taipan
Posts: 3909
Joined: Thu Feb 03, 2005 2:46 pm
Location: Canada

Post by canalon » Tue Nov 14, 2006 1:15 am

With GFP you do not need to fix your sample and to permeate membrane to allow penetration of the Antibodies, thus avoiding artifacts. Some variants of GFP also have different stability from very lowto quite high, which can be useful if you are interested in time dynamics of a protein expression (you will use an unstable variant) or just locating a particular structure (you will use a stable variant).

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

Post Reply

Who is online

Users browsing this forum: kellyjohnson and 2 guests