Immunofluorescence GFP and CFP problems

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kartikv
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Immunofluorescence GFP and CFP problems

Post by kartikv » Fri Nov 10, 2006 7:36 am

Hello,
I am currently using a GFP and CFP vector from Clontech, but whenever I do immunofluorescence with the empty vectors, all I get is a bunch of GFP and CFP protein aggregates. I have seen this multiple times and so have several other people in the neighboring labs. I was wondering if anyone has had similar problems and have successfully managed to troubleshoot them, because I have not been able to figure the problem out.

Please, I really need some help with this problem.

Thanks,
Kartik

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