enzyme activity

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suey
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enzyme activity

Post by suey » Mon May 09, 2005 7:42 am

what is the common method to analyze enzyme activity?

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biostudent84
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Post by biostudent84 » Mon May 09, 2005 3:18 pm

Nearly every chemical reaction in an organism is controlled by an enzyme. Usually these reactions are studied.

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thank.darwin
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Post by thank.darwin » Tue May 10, 2005 9:05 pm

biostudent84 wrote:Nearly every chemical reaction in an organism is controlled by an enzyme. Usually these reactions are studied.



I think that he is asking for a specific test or method?
No amount of experimentation can ever prove me right; a single experiment can prove me wrong.
-Albert Einstein

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biostudent84
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Post by biostudent84 » Tue May 10, 2005 11:20 pm

Benedict's solution is the best way to test for glucase activity.

There are different tests for different enzymes, so specifity is required =P

suey
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Post by suey » Thu May 12, 2005 7:35 am

i was trying to do the beta-mannanase activity that produced by fungi.

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Post by sashell4817 » Wed Feb 24, 2010 3:18 pm

how do you obtain evidence to support the suggestion that an experiment is an enzyme controlled reaction using 0.5M, 1.0M and 2.0M of hydrogen peroxide and potato tissue? oh the apparatus is setup to collect and count the amount of hydrogen bubbles being given off,thanks,your help is greatly appreciated
sash

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JackBean
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Post by JackBean » Wed Feb 24, 2010 7:46 pm

Measure activity and use blank reaction with inactivated enzymes (e.g. by boiling or using extreme pH), if you use enough low amount of the enzyme, you can also show enzyme saturation with substrate (but with only three concentrations that will be little hard...)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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