klenow fragment

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kabuto
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klenow fragment

Post by kabuto » Wed Nov 01, 2006 4:09 pm

i read that klenow fragment do not have 5'->3' exonuclease activity.

so, how does this help it to be used in random priming??

druid
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Post by druid » Wed Nov 01, 2006 4:20 pm

Polymerase with 5`->3` exonuclease activity may shorten primers. Although its polymerase activity is stronger than exonuclease, it may remove dna primers without synthesisng new ones.

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