Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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- Joined: Sun Apr 02, 2006 7:30 am
i read that klenow fragment do not have 5'->3' exonuclease activity.
so, how does this help it to be used in random priming??
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Polymerase with 5`->3` exonuclease activity may shorten primers. Although its polymerase activity is stronger than exonuclease, it may remove dna primers without synthesisng new ones.
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