isolation of purification of recombiant puc18 HELP!!!

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nextbigthing
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isolation of purification of recombiant puc18 HELP!!!

Post by nextbigthing » Wed Oct 25, 2006 2:10 pm

heres the thing I went to my lecturer for help on this and he just dismissed me and told me to go read a book. I have looked in many books for this and still don't know what I'm doing or what I'm lookin for.I really need help on this I've spent loads of time on this and have got nowhere.

ok...what would you expect to see on an electrophoresis photograph of running a hyperladder (known standard size of dna marker) next to recmobinant puc18 plasmid? And from the bands of this puc18 recombinant dna how can determine the purity of the dna?

I'm totally lost on this so I would really appreciate some help.

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